Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
CD80/B7-1 Antibody (1007-8)
Immunogen
Peptide
Host
Rabbit
Positive Control
Human brain tissue lysates, N2A, SHG-44, human tonsil tissue, mouse lung tissue, mouse spleen tissue, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified
MOLECULAR WEIGHT
60 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:100-1:500
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CD80/B7-1
SYNONYMS
Activation B7-1 antigen antibody; B lymphocyte activation antigen B7 antibody; B7 antibody; B7-1 antibody; B7-1 antigen antibody; B7.1 antibody; BB1 antibody; CD28 antigen ligand 1 antibody; CD28LG antibody; CD28LG1 antibody; CD80 antibody; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody; CD80 antigen antibody; CD80 molecule antibody; CD80_HUMAN antibody; Costimulatory factor CD80 antibody; costimulatory molecule variant IgV-CD80 antibody; CTLA-4 counter-receptor B7.1 antibody; LAB7 antibody; T-lymphocyte activation antigen CD80 antibody
TISSUE SPECIFICITY
Expressed on activated B-cells, macrophages and dendritic cells.
SUBCELLULAR LOCATION
Cell membrane
FUNCTION
B7-1 is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines. B7-1 is a costimulatory molecule for the activation of both CD4+ and CD8+ T lymphocytes that prevents the induction of clonal anergy. Thus, the transfer of B7-1 genes into tumor cells can induce protective immunity and lead to tumor rejection of some tumors in model systems of in vivo tumor growth.