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PRODUCT CODE: 1007-8

CD80/B7-1 Antibody (1007-8)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.
  • Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.
  • ICC staining CD80 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. <br />
  • ICC staining CD80 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. <br />
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD80 antibody. Counter stained with hematoxylin. <br />
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD80 antibody. Counter stained with hematoxylin. <br />
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD80 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with CD80 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD80/B7-1 Antibody (1007-8)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human brain tissue lysates, N2A, SHG-44, human tonsil tissue, mouse lung tissue, mouse spleen tissue, Hela.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P33681

PROTEIN NAME

T-lymphocyte activation antigen CD80

GENE NAME

CD80

SYNONYMS

B7

TISSUE SPECIFICITY

Expressed on activated B-cells, macrophages and dendritic cells.

SUBCELLULAR LOCATION

Membrane; Single-pass type I membrane protein.

FUNCTION

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.; (Microbial infection) Acts as a receptor for adenovirus subgroup B.