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PRODUCT CODE: 1007-8

CD80/B7-1 Antibody (1007-8)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.
  • Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.
  • ICC staining CD80 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD80 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD80 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD80 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD80 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with CD80 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD80/B7-1 Antibody (1007-8)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human brain tissue lysates, N2A, SHG-44, human tonsil tissue, mouse lung tissue, mouse spleen tissue, Hela.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P33681

PROTEIN NAME

CD80/B7-1

SYNONYMS

Activation B7-1 antigen antibody; B lymphocyte activation antigen B7 antibody; B7 antibody; B7-1 antibody; B7-1 antigen antibody; B7.1 antibody; BB1 antibody; CD28 antigen ligand 1 antibody; CD28LG antibody; CD28LG1 antibody; CD80 antibody; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody; CD80 antigen antibody; CD80 molecule antibody; CD80_HUMAN antibody; Costimulatory factor CD80 antibody; costimulatory molecule variant IgV-CD80 antibody; CTLA-4 counter-receptor B7.1 antibody; LAB7 antibody; T-lymphocyte activation antigen CD80 antibody

TISSUE SPECIFICITY

Expressed on activated B-cells, macrophages and dendritic cells.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

B7-1 is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines. B7-1 is a costimulatory molecule for the activation of both CD4+ and CD8+ T lymphocytes that prevents the induction of clonal anergy. Thus, the transfer of B7-1 genes into tumor cells can induce protective immunity and lead to tumor rejection of some tumors in model systems of in vivo tumor growth.