PRODUCT CODE: ER1706-35

CD71 Antibody (ER1706-35)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

ICC staining CD71 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD71 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD71 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD71 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD71 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD71 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HL-60 cells with CD71 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
ICC staining CD71 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD71 Antibody (ER1706-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, MCF-7, PC-3M, human kidney tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, HL-60.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Transferrin receptor protein 1

GENE NAME

TFRC

SYNONYMS

TR, TfR, TfR1, Trfr, sTfR, TFRC

SEQUENCE SIMILARITIES

Belongs to the peptidase M28 family. M28B subfamily.

POST-TRANSLATIONAL MODIFICATION

N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.; Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; [Transferrin receptor protein 1, serum form]: Secreted.

FUNCTION

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake.; (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.