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PRODUCT CODE: 0805-6

CD62E Antibody (0805-6)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

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Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: MCF-7<br /> Lane 2: Raji
  • Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: MCF-7<br /> Lane 2: Raji
  • ICC staining CD62E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD62E in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD62E in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Jurkat cells with CD62E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: Raji

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD62E Antibody (0805-6)

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (klh-coupled) corresponding to a region of n-terminal residues of cd62e.

Host

Rabbit

Positive Control

Hela, MCF-7, Raji, HepG2, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

67kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P16581

PROTEIN NAME

CD62E

SYNONYMS

CD26E antibody; CD62 antigen-like family member E antibody; CD62E antibody; E selectin antibody; E-selectin antibody; ELAM antibody; ELAM-1 antibody; ELAM1 antibody; Endothelial adhesion molecule 1 antibody; Endothelial leukocyte adhesion molecule 1 antibody; ESEL antibody; LECAM2 antibody; Leukocyte-endothelial cell adhesion molecule 2 antibody; LYAM2_HUMAN antibody; Sele antibody; Selectin E antibody

SEQUENCE SIMILARITIES

Belongs to the selectin/LECAM family.

SUBCELLULAR LOCATION

Cell membrane. Membrane.

FUNCTION

CD62E, known as E-selectin, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x. P-selectin has a similar function, but is expressed on the endothelial cell surface within minutes as it is stored within the cell rather than produced on demand.

CITATIONS

  • Wang, Qianting et al.

    Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells. | Archives of Oral Biology [2019]