PRODUCT CODE: em1701-38

CD47 Monoclonal Antibody (EM1701-38)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD47 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-CD47 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with CD47 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD47 Monoclonal Antibody (EM1701-38)

Immunogen

Recombinant protein within human cd47 aa 19-141 (extracellular).

Host

Mouse

Positive Control

SH-SY5Y cell, human prostate tissue, mouse fallopian tube tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1A9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

35 kDa

Isotype

IgM

APPLICATION DILUTION

  • ICC

  • 1:50-1:100

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Leukocyte surface antigen CD47

GENE NAME

CD47

SYNONYMS

IAP, CD47, MER6

TISSUE SPECIFICITY

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein.

FUNCTION

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.