PRODUCT CODE: ER1902-02

CD45 Antibody (ER1902-02)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.<br />
Lane 1: Anti-CD45 Antibody (1/500). <br />
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
  • Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.<br />
Lane 1: Anti-CD45 Antibody (1/500). <br />
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
  • Western blot analysis of CD45 on Jurkat cell lysates using anti-CD45 antibody.<br />
Lane 1: Anti-CD45 Antibody (1/500). <br />
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
  • Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.
Lane 1: Anti-CD45 Antibody (1/500).
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD45 Antibody (ER1902-02)

Immunogen

Synthetic peptide within human cd45 aa 1-60.

Host

Rabbit

Positive Control

U937 cell lysates, Jurkat cell lysates, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size 147 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Receptor-type tyrosine-protein phosphatase C

GENE NAME

PTPRC

SYNONYMS

PTPRC

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

TISSUE SPECIFICITY

Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.

DEVELOPMENTAL STAGE

Isoform 1: During T-cell development, expressed at the CD3-CD4-CD8- and CD3+CD4+CD8- stages but barely detectable at the CD3-CD4+CD8+ stage. Isoform 2: During T-cell development, expressed at low levels at the CD3-CD4-CD8- and CD3-CD4+CD8- stages but up-regulated at the CD3+CD4+CD8+ and CD3+CD4+CD8- stages. Isoform 3: During T-cell development, expressed at the CD3-CD4-CD8- and CD3+CD4+CD8- stages but barely detectable at the CD3-CD4+CD8+ stage. Isoform 5: During T-cell development, expressed at the CD3-CD4-CD8- and CD3+CD4+CD8- stages but barely detectable at the CD3-CD4+CD8+ stage. Isoform 7: Consistently expressed at high levels at all stages of T-cell development.

POST-TRANSLATIONAL MODIFICATION

Heavily N- and O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein. Note=Colocalized with DPP4 in membrane rafts.

FUNCTION

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).; (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.