PRODUCT CODE: EM010805

CD44 Monoclonal Antibody (EM010805)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of CD44 on different cell lysates using anti- CD44 antibody at 1/500 dilution.<br />
 Positive control: Line1: HUVEC Line2: HL-60 Line3: Hela Line4:SKOV3
  • Western blot analysis of CD44 on different cell lysates using anti- CD44 antibody at 1/500 dilution.<br />
 Positive control: Line1: HUVEC Line2: HL-60 Line3: Hela Line4:SKOV3
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with CD44 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Western blot analysis of CD44 on different cell lysates using anti- CD44 antibody at 1/500 dilution.
Positive control: Line1: HUVEC Line2: HL-60 Line3: Hela Line4:SKOV3

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD44 Monoclonal Antibody (EM010805)

Immunogen

Peptide

Host

Mouse

Positive Control

HUVEC, HL-6, Hela, SKOV3, human colon carcinoma tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A7-1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

80 kDa

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD44

SYNONYMS

LHR antibody; BA-1 antibody; CD 44 antibody; CD44 antibody; CD44 antigen antibody; CD44 molecule (Indian blood group) antibody; CD44 molecule antibody; CD44_HUMAN antibody; CDw44 antibody; Cell surface glycoprotein CD44 antibody; chondroitin sulfate proteoglycan 8 antibody; CSPG8 antibody; ECMR-III antibody; Epican antibody; Extracellular matrix receptor III antibody; GP90 lymphocyte homing/adhesion receptor antibody; HCELL antibody; hematopoietic cell E- and L-selectin ligand antibody; Heparan sulfate proteoglycan antibody; Hermes antigen antibody; homing function and Indian blood group system antibody; HSA antibody; HUTCH-I antibody; HUTCH1 antibody; Hyaluronate receptor antibody; IN antibody; INLU-related p80 Glycoprotein antibody; MC56 antibody; MDU2 antibody; MDU3 antibody; MGC10468 antibody; MIC4 antibody; MUTCH1 antibody; PGP-1 antibody; PGP-I antibody; PGP1 antibody; Phagocytic glycoprotein 1 antibody; Phagocytic glycoprotein I antibody; Soluble CD44 antibody

TISSUE SPECIFICITY

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

DEVELOPMENTAL STAGE

Expressed in the developing retina between 16 and 19 weeks post-conception, specifically in the outer neuroblastic zone, inner neuroblastic zone, interphotoreceptor zone and the retinal pigment epithelium (at protein level).

POST-TRANSLATIONAL MODIFICATION

Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.; N-glycosylated.; O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.; Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

CD44 is a cell-surface glycoprotein postulated to play a role in a variety of biological processes, including lymphocyte homing and tumor-cell metastasis. It mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA), and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications.