PRODUCT CODE: ER1803-54

CD40 Antibody (ER1803-54)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.
  • Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.
  • ICC staining CD40 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD40 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD40 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD40 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CD40 on Daudi cell lysate using anti-CD40 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD40 Antibody (ER1803-54)

Immunogen

Recombinant protein within human cd40 aa 10-250.

Host

Rabbit

Positive Control

Daudi, MG-63, SiHa, rat spleen tissue, human tonsil tissue, SKOV-3.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 31kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tumor necrosis factor receptor superfamily member 5

GENE NAME

CD40

SYNONYMS

CD40, TNFRSF5

TISSUE SPECIFICITY

B-cells and in primary carcinomas.

SUBCELLULAR LOCATION

[Isoform I]: Cell membrane; Single-pass type I membrane protein.; [Isoform II]: Secreted.

FUNCTION

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).