PRODUCT CODE: ER1706-80

CD4 Antibody (ER1706-80)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CD4 on different tissue lysates using anti-CD4 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse spleen <br />
  Lane 2: Mouse thymus
  • Western blot analysis of CD4 on different tissue lysates using anti-CD4 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse spleen <br />
  Lane 2: Mouse thymus
  • ICC staining CD4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with CD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of CD4 on different tissue lysates using anti-CD4 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse spleen
Lane 2: Mouse thymus

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD4 Antibody (ER1706-80)

Immunogen

Synthetic peptide within human cd4 aa 50-100.

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, mouse thymus tissue lysate, Hela, HepG2, SH-SY5Y, Jurkat, human tonsil tissue, human spleen tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CD4

SYNONYMS

CD 4 antibody; CD4 (L3T4) antibody; CD4 antibody; CD4 antigen (p55) antibody; CD4 antigen antibody; CD4 molecule antibody; CD4 receptor antibody; CD4+ Lymphocyte deficiency, included antibody; CD4_HUMAN antibody; CD4mut antibody; L3T4 antibody; Leu3 antibody; Ly-4 antibody; Lymphocyte antigen CD4 antibody; MGC165891 antibody; OTTHUMP00000238897 antibody; p55 antibody; T cell antigen T4 antibody; T cell antigen T4/LEU3 antibody; T cell differentiation antigen L3T4 antibody; T cell OKT4 deficiency, included antibody; T cell surface antigen T4/Leu 3 antibody; T cell surface antigen T4/Leu3 antibody; T cell surface glycoprotein CD4 antibody; T-cell surface antigen T4/Leu-3 antibody; T-cell surface glycoprotein CD4 antibody; W3/25 antibody; W3/25 antigen antibody

TISSUE SPECIFICITY

Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.; Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4 (also designated T4 and Leu 3), is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR in association with CD4 can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. An additional role of CD4 is to function as a receptor for HIV.