PRODUCT CODE: ER1706-66

CD34 Antibody (ER1706-66)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CD34 on TF-1 cell (1) and human brain tissue (2) lysates using anti-CD34 antibody at 1/1,000 dilution.
  • Western blot analysis of CD34 on TF-1 cell (1) and human brain tissue (2) lysates using anti-CD34 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD34 antibody. Counter stained with hematoxylin.
Western blot analysis of CD34 on TF-1 cell (1) and human brain tissue (2) lysates using anti-CD34 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD34 Antibody (ER1706-66)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

TF-1, human brain tissue lysate, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Hematopoietic progenitor cell antigen CD34

GENE NAME

CD34

SYNONYMS

CD34

SEQUENCE SIMILARITIES

Belongs to the CD34 family.

TISSUE SPECIFICITY

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

DEVELOPMENTAL STAGE

On early hematopoietic progenitor cells.

POST-TRANSLATIONAL MODIFICATION

Highly glycosylated.; Phosphorylated on serine residues by PKC.

SUBCELLULAR LOCATION

Membrane; Single-pass type I membrane protein.

FUNCTION

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.