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PRODUCT CODE: ER0802

CD34 Antibody (ER0802)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CD34 on different cell lysates using anti-CD34antibody at 1/1000 dilution.<br /> Positive control: <br /> Lane 1: Human brain <br /> Lane 2: Mouse brain <br /> Lane 3: Mouse testis <br /> Lane 4: Mouse thymus <br /> Lane 5: Mouse spleen <br /> Lane 6:Jurkat <br /> Lane 7: TF-1 <br /> Lane 8: F9
  • Western blot analysis of CD34 on different cell lysates using anti-CD34antibody at 1/1000 dilution.<br /> Positive control: <br /> Lane 1: Human brain <br /> Lane 2: Mouse brain <br /> Lane 3: Mouse testis <br /> Lane 4: Mouse thymus <br /> Lane 5: Mouse spleen <br /> Lane 6:Jurkat <br /> Lane 7: TF-1 <br /> Lane 8: F9
  • ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD34 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with CD34 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of CD34 on different cell lysates using anti-CD34antibody at 1/1000 dilution.
Positive control:
Lane 1: Human brain
Lane 2: Mouse brain
Lane 3: Mouse testis
Lane 4: Mouse thymus
Lane 5: Mouse spleen
Lane 6:Jurkat
Lane 7: TF-1
Lane 8: F9

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD34 Antibody (ER0802)

Immunogen

Synthetic peptide.

Host

Rabbit

Positive Control

Human brain, mouse brain, mouse testis, moue thymus, mouse spleen, Jurkat, TF-1, F9, human embryonic stem cell, human tonsil tissue, human liver carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:2,000-1:3,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P28906

PROTEIN NAME

CD34

SYNONYMS

CD34 antibody; CD34 antigen antibody; CD34 molecule antibody; CD34_HUMAN antibody; Cluster designation 34 antibody; Hematopoietic progenitor cell antigen CD34 antibody; HPCA1 antibody; Mucosialin antibody; OTTHUMP00000034733 antibody; OTTHUMP00000034734 antibody

SEQUENCE SIMILARITIES

Belongs to the CD34 family.

TISSUE SPECIFICITY

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

DEVELOPMENTAL STAGE

On early hematopoietic progenitor cells.

POST-TRANSLATIONAL MODIFICATION

Highly glycosylated.; Phosphorylated on serine residues by PKC.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.