PRODUCT CODE: 0108-2

CD34 Antibody (0108-2)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Human brain <br />
Lane 2: Mouse brain <br />
Lane 3: Mouse testis <br />
Lane 4: Mouse thymus <br />
Lane 5: Mouse spleen <br />
Lane 6:Jurkat <br />
Lane 7: TF-1 <br />
Lane 8: F9
  • Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Human brain <br />
Lane 2: Mouse brain <br />
Lane 3: Mouse testis <br />
Lane 4: Mouse thymus <br />
Lane 5: Mouse spleen <br />
Lane 6:Jurkat <br />
Lane 7: TF-1 <br />
Lane 8: F9
  • ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- CD34 rabbit polyclonal antibody.
Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1000 dilution.
Positive control:
Lane 1: Human brain
Lane 2: Mouse brain
Lane 3: Mouse testis
Lane 4: Mouse thymus
Lane 5: Mouse spleen
Lane 6:Jurkat
Lane 7: TF-1
Lane 8: F9

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD34 Antibody (0108-2)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human brain, mouse brain, mouse testis, Mouse thymus, mouse spleen, Jurkat, TF-1, F9, human embryonic stem cell, human tonsil tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100

  • IHC-P

  • 1:3,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Hematopoietic progenitor cell antigen CD34

GENE NAME

CD34

SEQUENCE SIMILARITIES

Belongs to the CD34 family.

TISSUE SPECIFICITY

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

DEVELOPMENTAL STAGE

On early hematopoietic progenitor cells.

POST-TRANSLATIONAL MODIFICATION

Highly glycosylated.; Phosphorylated on serine residues by PKC.

SUBCELLULAR LOCATION

Membrane; Single-pass type I membrane protein.

FUNCTION

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.