PRODUCT CODE: 0804-4

CD24 Antibody (0804-4)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis on human spleen using anti-CD24 polyclonal antibody
  • Western blot analysis on human spleen using anti-CD24 polyclonal antibody
  • ICC staining CD24 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis on human spleen using anti-CD24 polyclonal antibody

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD24 Antibody (0804-4)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human spleen,Hela

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

39kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:100-500

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Signal transducer CD24

GENE NAME

CD24

SYNONYMS

CD24

SEQUENCE SIMILARITIES

Belongs to the CD24 family.

TISSUE SPECIFICITY

B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

POST-TRANSLATIONAL MODIFICATION

Extensively O-glycosylated.

SUBCELLULAR LOCATION

Cell membrane; Lipid-anchor, GPI-anchor.

FUNCTION

May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity (By similarity).

CITATIONS

  • Xiang, Xiaocong et al.

    Tex10 promotes stemness and EMT phenotypes in esophageal squamous cell carcinoma via the Wnt/�_���catenin pathway. | Oncology Reports [2019]