PRODUCT CODE: EM1901-89

CD1A Mouse Monoclonal Antibody [A3F9] (EM1901-89)

  • IVD–IHC

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of CD1A on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD1A on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-89, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD1A on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD1A Mouse Monoclonal Antibody [A3F9] (EM1901-89)

Immunogen

Recombinant protein within human cd1a aa 50-250.

Host

Mouse

Positive Control

K562 cell lysates, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3F9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD1A

SYNONYMS

CD 1a antibody; CD1 antibody; CD1a antibody; CD1A Antigen antibody; CD1A antigen, a polypeptide antibody; CD1a molecule antibody; CD1A_HUMAN antibody; cluster of differentiation 1 A antibody; cortical thymocyte antigen CD1A antibody; differentiation antigen CD1 alpha 3 antibody; epidermal dendritic cell marker CD1a antibody; FCB 6 antibody; FCB6 antibody; HTA 1 antibody; HTA1 antibody; hTa1 thymocyte antigen antibody; OTTHUMP00000018907 antibody; R 4 antibody; R4 antibody; T 6 antibody; T-cell surface antigen T6/Leu-6 antibody; T-cell surface glycoprotein CD1a antibody; T6 antibody

TISSUE SPECIFICITY

Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.

SUBCELLULAR LOCATION

Cell membrane, endosome membrane, membrane raft.

FUNCTION

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants.