PRODUCT CODE: EM40308

CD19 Monoclonal Antibody (EM40308)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM40308, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Raji cell lysate<br />
Lane 2: Daudi cell lysate
  • Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM40308, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Raji cell lysate<br />
Lane 2: Daudi cell lysate
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40308, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of CD19 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM40308, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM40308, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD19 Monoclonal Antibody (EM40308)

Immunogen

Synthetic peptide within human cd19 110-190 aa.

Host

Mouse

Positive Control

Mouse lymphatic tissue, Raji, Daudi, human spleen tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3-A8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

61 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

B-lymphocyte antigen CD19

GENE NAME

CD19

SYNONYMS

CD19

TISSUE SPECIFICITY

Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.

FUNCTION

Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.

CITATIONS

  • Li, Ning et al.

    Tmem30a Plays Critical Roles in Ensuring the Survival of Hematopoietic Cells and Leukemia Cells in Mice. | The American Journal of Pathology [2018]