PRODUCT CODE: em1701-25

CD134/OX40 Monoclonal Antibody (EM1701-25)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HL-60 cells with CD134 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD134/OX40 Monoclonal Antibody (EM1701-25)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

A549, Hela, HepG2, human tonsil tissue, human spleen tissue, mouse spleen tissue, HL-60.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2H2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG1

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Tumor necrosis factor receptor superfamily member 4

GENE NAME

TNFRSF4

SYNONYMS

TNFRSF4, TXGP1L

SUBCELLULAR LOCATION

Membrane; Single-pass type I membrane protein.

FUNCTION

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.; (Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.