PRODUCT CODE: EM1701-25

CD134/OX40 Mouse Monoclonal Antibody [2H2] (EM1701-25)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HL-60 cells with CD134 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD134/OX40 Mouse Monoclonal Antibody [2H2] (EM1701-25)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

A549, Hela, HepG2, human tonsil tissue, human spleen tissue, mouse spleen tissue, HL-60.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2H2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG1

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

CD134/OX40

SYNONYMS

ACT 35 antibody; ACT35 antibody; ACT35 antigen antibody; ATC35 antigen antibody; CD 134 antibody; CD134 antibody; CD134 antigen antibody; IMD16 antibody; Lymphoid activation antigene ACT35 antibody; OX 40 antibody; OX40 antibody; OX40 antigen antibody; OX40 cell surface antigen antibody; OX40 homologue antibody; OX40L receptor antibody; TAX transcriptionally activated glycoprotein 1 receptor antibody; TAX transcriptionally-activated glycoprotein 1 receptor antibody; TNF receptor superfamily member 4 antibody; TNFRSF 4 antibody; TNFRSF4 antibody; TNR4_HUMAN antibody; Tumor necrosis factor receptor superfamily member 4 antibody; Txgp 1l antibody; Txgp1 antibody; Txgp1l antibody

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Ox40 (also designated CD134 and Ox40R), is a member of the tumor necrosis factor receptor (TNFR) family. Ox40 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. Ox40 is also involved in the stimulation of T cells, T cell-dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. Ox40 is expressed on activated CD4+ T lymphocytes and its ligand, Ox40L, is found preferentially on activated B cells. Engagement of Ox40 with its ligand, Ox40L, delivers a strong costimulatory signal to effector T cells. Members of the TNFR superfamily are critically involved in the regulation of infections, inflammation, autoimmune diseases and tissue homeostasis.