PRODUCT CODE: EM1701-28

CD133 Monoclonal Antibody (EM1701-28)

Applications

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD133 (green) in N2A cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD133 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD133 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.
ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD133 Monoclonal Antibody (EM1701-28)

Immunogen

Peptide

Host

Mouse

Positive Control

LOVO, N2A, human colon cancer tissue, human uterus tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

D2-B1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

Predicted band size 97 kDa

Isotype

IgG1

APPLICATION DILUTION

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD133

SYNONYMS

AC133 antibody; Antigen AC133 antibody; CD133 antibody; CORD12 antibody; Hematopoietic stem cell antigen antibody; hProminin antibody; MCDR2 antibody; MSTP061 antibody; OTTHUMP00000217744 antibody; OTTHUMP00000217745 antibody; OTTHUMP00000217746 antibody; PROM1 antibody; PROM1_HUMAN antibody; Prominin I antibody; Prominin like 1 antibody; Prominin like protein 1 precursor antibody; Prominin mouse like 1 antibody; Prominin-1 antibody; Prominin-like protein 1 antibody; Prominin1 antibody; PROML1 antibody; RP41 antibody; STGD4 antibody

SEQUENCE SIMILARITIES

Belongs to the prominin family.

TISSUE SPECIFICITY

Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Isoform 1 and isoform 2 are glycosylated.; Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Plasma membrane. Cell projection.

FUNCTION

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. While the precise function of CD133 remains unknown, it has been proposed to act as an organizer of cell membrane topology.

CITATIONS

  • Ye, Sen et al.

    SET Domain-Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence. | Cancer Research [2019]