CD11b Mouse Monoclonal Antibody [A2-9] (M1211-6)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.

Applications

IHC-P
FC

REACTIVITY

Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD11b Mouse Monoclonal Antibody [A2-9] (M1211-6)

Immunogen

Recombinant protein within human cd11b 500-750 aa.

Host

Mouse

Positive Control

Human tonsil, Raji, human spleen

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

130kDa

Isotype

IgG2b

APPLICATION DILUTION

IHC-P
1:200

FC
1:200

TARGET

UNIPROT #

P11215

PROTEIN NAME

CD11b

SYNONYMS

antigen CD11b (p170) antibody; Antigen CD11b p170 antibody; CD11 antigen like family member B antibody; CD11 antigen-like family member B antibody; CD11b antibody; CD11b/CD18 antibody; CD49d antibody; Cell surface glycoprotein MAC-1 subunit alpha antibody; Complement component 3 receptor 3 subunit antibody; Complement Component Receptor 3 Alpha antibody; Complement receptor type 3, alpha subunit antibody; CR 3 alpha chain (CR3A) antibody; CR 3 alpha chain antibody; CR-3 alpha chain antibody; CR3 antibody; CR3A antibody; F730045J24Rik antibody; Integrin Alpha M antibody; Integrin alpha M chain antibody; Integrin alpha-M antibody; Integrin beta 2 alpha subunit antibody; Integrin subunit alpha M antibody; integrin, alpha M (complement component 3 receptor 3 subunit) antibody; ITAM_HUMAN antibody; ITGAM antibody; Leukocyte adhesion receptor MO1 antibody; Ly-40 antibody; MAC 1 antibody; Mac-1a antibody; MAC1 antibody; Mac1, alpha subunit antibody; MAC1A antibody; Macrophage antigen alpha polypeptide antibody; MGC117044 antibody; Mo1, alpha subunit antibody; MO1A antibody; Neutrophil adherence receptor alpha M subunit antibody; Neutrophil adherence receptor antibody; SLEB6 antibody

SEQUENCE SIMILARITIES

Belongs to the integrin alpha chain family.

TISSUE SPECIFICITY

Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).

SUBCELLULAR LOCATION

Membrane

FUNCTION

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY

The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)

Applications

REACTIVITY

Human

Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat