PRODUCT CODE: M1004-3

CCDC47 Mouse Monoclonal Antibody [1-B9-5] (M1004-3)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of CCDC47 on recombinant protein using anti-CCDC47 antibody at 1/1,000 dilution.
  • Western blot analysis of CCDC47 on recombinant protein using anti-CCDC47 antibody at 1/1,000 dilution.
  • ICC staining CCDC47 in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CCDC47 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
Western blot analysis of CCDC47 on recombinant protein using anti-CCDC47 antibody at 1/1,000 dilution.

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CCDC47 Mouse Monoclonal Antibody [1-B9-5] (M1004-3)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Recombinant protein, F9, MCF-7, human liver tissue, human lung tissue, human testis tissue, human breast tissue, human colon tissue, human stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-B9-5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

MOLECULAR WEIGHT

56 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CCDC47

SYNONYMS

CCD47_HUMAN antibody; CCDC 47 antibody; CCDC47 antibody; Coiled coil domain containing 47 antibody; Coiled coil domain containing protein 47 antibody; Coiled-coil domain-containing protein 47 antibody; GK001 antibody; MSTP041 antibody

SUBCELLULAR LOCATION

Membrane, nucleus.

FUNCTION

Coiled-coil domain 47 (CCDC47) is a gene located on human chromosome 17, specifically locus 17q23.3 which encodes for the protein CCDC47. The gene has several aliases including GK001 and MSTP041. The protein itself contains coiled-coil domains, the SEEEED superfamily, a domain of unknown function (DUF1682) and a transmembrane domain. The function of the protein is unknown, but it has been proposed that CCDC47 is involved in calcium ion homeostasis and the endoplasmic reticulum overload response. In addition to the high levels of phosphorylation seen in CCDC47, three sulfination sites are predicted and conserved in mammals, reptiles and birds but not in fish, amphibians or invertebrates. Five potential sumoylation sites are also seen and conserved back to the bony fish. There is no glycosylation of the protein as it is not predicted to extend into the extracellular portion of the cell.