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PRODUCT CODE: ET7109-21

CBS Recombinant Rabbit Monoclonal Antibody [JE40-99] (ET7109-21)

  • Recombinant

Applications

  • WB

  • ICC

REACTIVITY

  • Human

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Western blot analysis of CBS on different lysates using anti-CBS antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: 293 <br /> Lane 2: 293<br /> Lane 3: human liver tissue
  • Western blot analysis of CBS on different lysates using anti-CBS antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: 293 <br /> Lane 2: 293<br /> Lane 3: human liver tissue
  • ICC staining CBS in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of CBS on different lysates using anti-CBS antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: 293
Lane 3: human liver tissue

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CBS Recombinant Rabbit Monoclonal Antibody [JE40-99] (ET7109-21)

Immunogen

Recombinant protein within human cbs aa 400-550.

Host

Rabbit

Positive Control

293, human liver tissue lysate, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE40-99

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

61 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

TARGET

UNIPROT #

P35520

PROTEIN NAME

CBS

SYNONYMS

AI047524 antibody; AI303044 antibody; Beta thionase antibody; Beta-thionase antibody; Cbs antibody; Cbs cystathionine beta-synthase antibody; CBS_HUMAN antibody; Cystathionine beta synthase antibody; Cystathionine beta-synthase antibody; EC 4.2.1.22 antibody; HIP 4 antibody; HIP4 antibody; Methylcysteine synthase antibody; MGC18856 antibody; MGC18895 antibody; MGC37300 antibody; OTTHUMP00000109416 antibody; OTTHUMP00000109418 antibody; Serine sulfhydrase antibody

SEQUENCE SIMILARITIES

Belongs to the cysteine synthase/cystathionine beta-synthase family.

TISSUE SPECIFICITY

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Strongly expressed in human liver and pancreas, with weaker expression in heart and brain, the cytoplasmic protein cystathionine b-synthase (CBS) operates in the first step of homocysteine transulfuration. CBS, which belongs to the cysteine synthase/cystathionine b-synthase family of proteins, catalyzes the formation of cystathionine from the thrombogenic amino acid homocysteine using pyridoxal phosphate cofactor. Allosteric activation by adenosyl-methionine regulates CBS activity. Deficiencies in CBS are assoc-iated with homocystinuria, a recessively inherited error in sulfur amino acid metabolism that affects many organs and tissues. Symptoms of homocytinuria include arteriosclerosis, thrombosis, dislocated optic lenses, mental retard-ation and skeletal abnormalities.