PRODUCT CODE: EM40728

Caveolin-1 Monoclonal Antibody (EM40728)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Caveolin-1 on different cell lysates using anti-Caveolin-1 antibody at 1/500 dilution.
  • Western blot analysis of Caveolin-1 on different cell lysates using anti-Caveolin-1 antibody at 1/500 dilution.
  • ICC staining Caveolin-1 in L929 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
Western blot analysis of Caveolin-1 on different cell lysates using anti-Caveolin-1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Caveolin-1 Monoclonal Antibody (EM40728)

Immunogen

Peptide

Host

Mouse

Positive Control

Hela, HepG2, A549, Jurkat, F9, L929, human lung tissue, mouse lung tissue, mouse liver tissue, rat lung tissue, mouse bladder tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

19-D6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:200

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Caveolin-1

GENE NAME

CAV1

SYNONYMS

CAV1

SEQUENCE SIMILARITIES

Belongs to the caveolin family.

TISSUE SPECIFICITY

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

SUBCELLULAR LOCATION

Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Golgi apparatus, trans-Golgi network. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

FUNCTION

May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.