PRODUCT CODE: EM40728

Caveolin-1 Monoclonal Antibody (EM40728)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Caveolin-1 on different cell lysates using anti-Caveolin-1 antibody at 1/500 dilution.
  • Western blot analysis of Caveolin-1 on different cell lysates using anti-Caveolin-1 antibody at 1/500 dilution.
  • ICC staining Caveolin-1 in L929 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
Western blot analysis of Caveolin-1 on different cell lysates using anti-Caveolin-1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Caveolin-1 Monoclonal Antibody (EM40728)

Immunogen

Peptide

Host

Mouse

Positive Control

Hela, HepG2, A549, Jurkat, F9, L929, human lung tissue, mouse lung tissue, mouse liver tissue, rat lung tissue, mouse bladder tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

19-D6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:200

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Caveolin-1

SYNONYMS

BSCL3 antibody; CAV antibody; CAV1 antibody; CAV1_HUMAN antibody; caveolae protein, 22 kD antibody; caveolin 1 alpha isoform antibody; caveolin 1 beta isoform antibody; Caveolin 1 caveolae protein 22kDa antibody; Caveolin-1 antibody; Caveolin1 antibody; cell growth-inhibiting protein 32 antibody; CGL3 antibody; LCCNS antibody; MSTP085 antibody; OTTHUMP00000025031 antibody; PPH3 antibody; VIP 21 antibody; VIP21 antibody

SEQUENCE SIMILARITIES

Belongs to the caveolin family.

TISSUE SPECIFICITY

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

Caveolin, an integral membrane protein, is a principal component of caveolae membranes in vivo. Two isoforms of caveolin have been identified: a slower migrating 24-kDa species (-isoform) and a faster migrating 21-kDa species (-isoform). Caveolins interact with multiple signaling molecules, such as the G-protein alpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS. Caveolin-1 has been implicated in the pathogenesis of mammary epithelial cell hyperplasia.