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PRODUCT CODE: ER40125

Catalase Antibody (ER40125)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Caveolin-1 on different lysates using anti-Caveolin-1 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: F9<br /> Lane 2: A549<br /> Lane 3: Mouse lung<br /> Lane 4: Mouse liver<br /> Lane 5: Human lung<br /> Lane 6: Hela<br /> Lane 7: HepG2<br /> Lane 8: Jurkat
  • Western blot analysis of Caveolin-1 on different lysates using anti-Caveolin-1 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: F9<br /> Lane 2: A549<br /> Lane 3: Mouse lung<br /> Lane 4: Mouse liver<br /> Lane 5: Human lung<br /> Lane 6: Hela<br /> Lane 7: HepG2<br /> Lane 8: Jurkat
  • ICC staining catalase in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining catalase in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-catalase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-catalase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-catalase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-catalase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-catalase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-catalase antibody. Counter stained with hematoxylin.
Western blot analysis of Caveolin-1 on different lysates using anti-Caveolin-1 antibody at 1/500 dilution.
Positive control:
Lane 1: F9
Lane 2: A549
Lane 3: Mouse lung
Lane 4: Mouse liver
Lane 5: Human lung
Lane 6: Hela
Lane 7: HepG2
Lane 8: Jurkat

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Catalase Antibody (ER40125)

Immunogen

Synthetic peptide (klh-coupled) within human caveolin-1 110-180aa.

Host

Rabbit

Positive Control

Hela, HepG2, A431, mouse liver tissue, mouse kidney tissue, human liver tissue, rat kidney tissue, rat liver tissue, rat lung tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

P04040

PROTEIN NAME

Catalase

GENE NAME

CAT

SYNONYMS

CATA

SEQUENCE SIMILARITIES

Belongs to the catalase family.

POST-TRANSLATIONAL MODIFICATION

The N-terminus is blocked.

SUBCELLULAR LOCATION

Peroxisome.

FUNCTION

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

CITATIONS

  • Li, Kaiqun et al.

    High cholesterol inhibits tendon-related gene expressions in tendon-derived stem cells through reactive oxygen species-activated nuclear factor-�B signaling. | Journal of Cellular Physiology [2019]