Rabbit polyclonal primary
Caspase-3 Rabbit Polyclonal Antibody (ER1802-42)
Synthetic peptide within human caspase-3 aa 150-175.
A549, HepG2, HUVEC, Jurkat, human tonsil tissue, human spleen tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified
A830040C14Rik antibody; Apopain antibody; CASP-3 antibody; CASP3 antibody; CASP3_HUMAN antibody; Casp3a antibody; Caspase 3 antibody; Caspase 3, apoptosis-related cysteine peptidase antibody; Caspase 3, apoptosis-related cysteine protease antibody; Caspase 3, apoptosis-related cysteine protease a antibody; Caspase-3 subunit p12 antibody; CC3 antibody; CPP-32 antibody; CPP32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; EC 22.214.171.124 antibody; LICE antibody; mldy antibody; OTTHUMP00000165052 antibody; OTTHUMP00000165053 antibody; OTTHUMP00000165054 antibody; PARP cleavage protease antibody; Procaspase3 antibody; Protein Yama antibody; SCA 1 antibody; SCA-1 antibody; SREBP cleavage activity 1 antibody; Yama antibody
Belongs to the peptidase C14A family.
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.