PRODUCT CODE: ER1803-62

CASK Antibody (ER1803-62)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: SiHa<br />
 Lane 2: A549<br />
  • Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: SiHa<br />
 Lane 2: A549<br />
  • ICC staining CASK in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CASK antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CASK antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CASK antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: A549

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CASK Antibody (ER1803-62)

Immunogen

Recombinant protein within human cask aa 300-500.

Host

Rabbit

Positive Control

SiHa, A549, HT-29, rat brain tissue, human appendix tissue, human prostate cancer tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

105/59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Peripheral plasma membrane protein CASK

GENE NAME

CASK

SYNONYMS

hCASK, CASK, LIN2

SEQUENCE SIMILARITIES

In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.; Belongs to the MAGUK family.

TISSUE SPECIFICITY

Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein.

FUNCTION

Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Component of the LIN-10-LIN-2-LIN-7 complex, which associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity).