Cardiac Troponin /TTNNT2 Antibody (ER21102)

Western blot analysis of TNNT2 on different lysates using anti-TNNT2 antibody at 1/5,000 dilution.

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cardiac Troponin /TTNNT2 Antibody (ER21102)

Immunogen

Synthetic peptide (klh-coupled) within human tnnt2 1-100 aa.

Host

Rabbit

Positive Control

Human heart tissue, human skeletal muscle tissue, mouse heart tissue, rat heart tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:5000

IHC-P
1:200

TARGET

UNIPROT #

P45379

PROTEIN NAME

Troponin T, cardiac muscle

GENE NAME

TNNT2

SYNONYMS

TnTc,cTnT

SEQUENCE SIMILARITIES

Belongs to the troponin T family.

TISSUE SPECIFICITY

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

FUNCTION

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.