PRODUCT CODE: ER21102

Cardiac Troponin /TTNNT2 Antibody (ER21102)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of TNNT2 on different lysates using anti-TNNT2 antibody at 1/5,000 dilution.
  • Western blot analysis of TNNT2 on different lysates using anti-TNNT2 antibody at 1/5,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.
Western blot analysis of TNNT2 on different lysates using anti-TNNT2 antibody at 1/5,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cardiac Troponin /TTNNT2 Antibody (ER21102)

Immunogen

Synthetic peptide (klh-coupled) within human tnnt2 1-100 aa.

Host

Rabbit

Positive Control

Human heart tissue, human skeletal muscle tissue, mouse heart tissue, rat heart tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5000

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Cardiac Troponin /TTNNT2

SYNONYMS

Cardiac muscle troponin T antibody; Cardiomyopathy dilated 1D (autosomal dominant) antibody; Cardiomyopathy hypertrophic 2 antibody; CMD1D antibody; CMH2 antibody; CMPD2 antibody; cTnT antibody; LVNC6 antibody; MGC3889 antibody; OTTHUMP00000033864 antibody; OTTHUMP00000033865 antibody; OTTHUMP00000033866 antibody; OTTHUMP00000033867 antibody; OTTHUMP00000033870 antibody; OTTHUMP00000218095 antibody; RCM3 antibody; TNNT 2 antibody; TNNT2 antibody; TNNT2_HUMAN antibody; TnTc antibody; Troponin T cardiac muscle antibody; Troponin T type 2 (cardiac) antibody; Troponin T type 2 cardiac antibody; Troponin T, cardiac muscle antibody; Troponin T2 cardiac antibody

SEQUENCE SIMILARITIES

Belongs to the troponin T family.

TISSUE SPECIFICITY

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

SUBCELLULAR LOCATION

Cytosol

FUNCTION

Troponin T type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations of this gene may be associated with mild or absent hypertrophy and predominant restrictive disease, with a high risk of sudden cardiac death.