Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Cardiac Troponin /TTNNT2 Antibody (ER21102)
Immunogen
Synthetic peptide (klh-coupled) within human tnnt2 1-100 aa.
Host
Rabbit
Positive Control
Human heart tissue, human skeletal muscle tissue, mouse heart tissue, rat heart tissue
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
36 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:5000
-
IHC-P
-
1:200
TARGET
UNIPROT #
PROTEIN NAME
Cardiac Troponin /TTNNT2
SYNONYMS
Cardiac muscle troponin T antibody; Cardiomyopathy dilated 1D (autosomal dominant) antibody; Cardiomyopathy hypertrophic 2 antibody; CMD1D antibody; CMH2 antibody; CMPD2 antibody; cTnT antibody; LVNC6 antibody; MGC3889 antibody; OTTHUMP00000033864 antibody; OTTHUMP00000033865 antibody; OTTHUMP00000033866 antibody; OTTHUMP00000033867 antibody; OTTHUMP00000033870 antibody; OTTHUMP00000218095 antibody; RCM3 antibody; TNNT 2 antibody; TNNT2 antibody; TNNT2_HUMAN antibody; TnTc antibody; Troponin T cardiac muscle antibody; Troponin T type 2 (cardiac) antibody; Troponin T type 2 cardiac antibody; Troponin T, cardiac muscle antibody; Troponin T2 cardiac antibody
SEQUENCE SIMILARITIES
Belongs to the troponin T family.
TISSUE SPECIFICITY
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
SUBCELLULAR LOCATION
Cytosol
FUNCTION
Troponin T type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations of this gene may be associated with mild or absent hypertrophy and predominant restrictive disease, with a high risk of sudden cardiac death.