PRODUCT CODE: em1701-61

Calreticulin Monoclonal Antibody (EM1701-61)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.
  • Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.
  • ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Calreticulin Monoclonal Antibody (EM1701-61)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

HL-60, A431, HepG2, human thyroid gland tissue, human prostate cancer tissue, human placenta tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7B2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Calreticulin

GENE NAME

CALR

SYNONYMS

ERp60, CALR, CRTC

SEQUENCE SIMILARITIES

Belongs to the calreticulin family.

SUBCELLULAR LOCATION

Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Sarcoplasmic reticulum lumen.

FUNCTION

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).