PRODUCT CODE: ER1803-28

CACNG5 Antibody (ER1803-28)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of CACNG5 on LOVO cell lysate using anti-CACNG5 antibody at 1/200 dilution.
  • Western blot analysis of CACNG5 on LOVO cell lysate using anti-CACNG5 antibody at 1/200 dilution.
  • ICC staining CACNG5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CACNG5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CACNG5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of PANC-1 cells with CACNG5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CACNG5 on LOVO cell lysate using anti-CACNG5 antibody at 1/200 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CACNG5 Antibody (ER1803-28)

Immunogen

Synthetic peptide within rat cacng5 aa 60-110.

Host

Rabbit

Positive Control

HepG2, LOVO, PANC-1.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

31 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

CACNG5

SYNONYMS

CACNG 5 antibody; Cacng5 antibody; Calcium channel voltage dependent gamma subunit 5 antibody; CCG5_HUMAN antibody; MGC126656 antibody; MGC126682 antibody; Neuronal voltage gated calcium channel gamma 5 subunit antibody; Neuronal voltage-gated calcium channel gamma-5 subunit antibody; Voltage dependent calcium channel gamma 5 subunit antibody; Voltage-dependent calcium channel gamma-5 subunit antibody

SEQUENCE SIMILARITIES

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

SUBCELLULAR LOCATION

Plasma membrane.

FUNCTION

Regulates the gating properties of AMPA-selective glutamate receptors (AMPARs). Modulates their gating properties by accelerating their rates of activation, deactivation and desensitization. Displays subunit-specific AMPA receptor regulation. Shows specificity for GRIA1, GRIA4 and the long isoform of GRIA2. Thought to stabilize the calcium channel in an inactivated (closed) state.