PRODUCT CODE: ER1803-13

Cacng4 Antibody (ER1803-13)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.
  • Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.
  • ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cacng4 Antibody (ER1803-13)

Immunogen

Synthetic peptide within rat cacng4 aa 150-250.

Host

Rabbit

Positive Control

MCF-7, SH-SY-5Y, rat testis tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Cacng4

SYNONYMS

CACNG4 antibody; Calcium channel voltage dependent gamma subunit 4 antibody; CCG4_HUMAN antibody; Neuronal voltage gated calcium channel gamma 4 subunit antibody; Neuronal voltage-gated calcium channel gamma-4 subunit antibody; TARP gamma-4 antibody; Transmembrane AMPAR regulatory protein gamma-4 antibody; Voltage dependent calcium channel gamma 4 subunit antibody; Voltage-dependent calcium channel gamma-4 subunit antibody

SEQUENCE SIMILARITIES

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

TISSUE SPECIFICITY

Detected in heart left ventricle.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit. Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs), including GRIA1 and GRIA4. Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization.