Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: HUVEC cell lysate
Applications
-
WB
-
ICC
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
C7 Mouse Monoclonal Antibody [15D1] (EM1901-14)
Immunogen
Recombinant protein within human c7 aa 498-641 / 843.
Host
Mouse
Positive Control
HepG2 cell, K562 cell, HUVEC cell, HCT116 cell, SHG-44 cell.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
15D1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
94 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
ICC:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
C7
SYNONYMS
C7 antibody; CO7_HUMAN antibody; complement component 7 antibody; Complement component C7 antibody
SEQUENCE SIMILARITIES
Belongs to the complement C6/C7/C8/C9 family.
POST-TRANSLATIONAL MODIFICATION
C7 has 28 disulfide bridges.; C-, N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
This gene encodes a serum glycoprotein that forms a membrane attack complex together with complement components C5b, C6, C8, and C9 as part of the terminal complement pathway of the innate immune system. Its primary task is to bind the C5bC6 complex together. This junction alters the configuration of the protein molecules, exposing a hydrophobic site on C7 that allows the C7 to insert into the phospholipid bilayer of the pathogen. The protein encoded by this gene contains a cholesterol-dependent cytolysin/membrane attack complex/perforin-like (CDC/MACPF) domain and belongs to a large family of structurally related molecules that form pores involved in host immunity and bacterial pathogenesis. This protein initiates membrane attack complex formation by binding the C5b-C6 subcomplex and inserts into the phospholipid bilayer, serving as a membrane anchor. Mutations in this gene are associated with a rare disorder called C7 deficiency.