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c-Fos Recombinant Rabbit Monoclonal Antibody [PSH07-51] (HA722839)

Price: Regular price $385.00

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Catalog# HA722839

Rabbit Monoclonal to c-Fos

Tested for

  • WB

  • IHC-Fr

  • IF-Cell

  • IHC-P

  • ChIP

  • IF-Tissue

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

  • Cynomolgus monkey

  • Pig

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

c-Fos Recombinant Rabbit Monoclonal Antibody [PSH07-51] (HA722839)

Immunogen

Recombinant protein within human protein c-fos aa 1-380.

Host

Rabbit

Positive Control

Human brain tissue, mouse brain tissue, mouse hippocampus tissue, HeLa cells serum starved for 40 hours then add 20% FBS for 2 hours, RAW264.7 cells serum starved for 16 hours then add 20% FBS for 4 hours, C6 cells serum starved for 16 hours then add 10% FBS for 30 minutes, HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate, RAW264.7 serum starved for 16 hours then add 200nM PMA for 4 hours cell lysate, C6 serum starved for 16 hours then add 10% FBS for 30 minutes cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PSH07-51

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IHC-Fr

  • 1:4,000-1:10,000

  • IF-Cell

  • 1:1,000-1:2,000

  • IHC-P

  • 1:5,000

  • ChIP

  • Use 0.5~2 μg for 25 μg of chromatin.

  • IF-Tissue

  • 1:4000-1:10,000

  • IP

  • 1-2μg/sample

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 41 kDa

Isotype

IgG

TARGET

UNIPROT #

P01100

PROTEIN NAME

c-Fos

SYNONYMS

Activator protein 1 antibody;AP 1 antibody;C FOS antibody;Cellular oncogene c fos antibody;Cellular oncogene fos antibody;FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody;FBJ murine osteosarcoma viral oncogene homolog antibody;FBJ murine osteosarcoma viral v fos oncogene homolog antibody;FBJ Osteosarcoma Virus antibody;FOS antibody;FOS protein antibody;FOS_HUMAN antibody;G0 G1 switch regulatory protein 7 antibody;G0/G1 switch regulatory protein 7 antibody;G0S7 antibody;Oncogene FOS antibody;p55 antibody;proto oncogene c Fos antibody;Proto oncogene protein c fos antibody;Proto-oncogene c-Fos antibody;v fos FBJ murine osteosarcoma viral oncogene homolog antibody

SUBCELLULAR LOCATION

Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.

FUNCTION

Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2. It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression. It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.

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