PRODUCT CODE: ER1802-66

BNIP1 Antibody (ER1802-66)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.
  • Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.
  • ICC staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.
Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BNIP1 Antibody (ER1802-66)

Immunogen

Recombinant protein with human bn1p1 aa 1-220.

Host

Rabbit

Positive Control

Rat liver tissue lysate, HepG2, LOVO, human liver tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:100

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vesicle transport protein SEC20

GENE NAME

BNIP1

SYNONYMS

TRG-8, BNIP1, NIP1, SEC20L

SEQUENCE SIMILARITIES

Belongs to the SEC20 family.

TISSUE SPECIFICITY

Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Single-pass type IV membrane protein; Single-pass type IV membrane protein.

FUNCTION

As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Plays also a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).