PRODUCT CODE: ER80602

BMP2 Antibody (ER80602)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of BMP2 on different lysates using anti-BMP2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat kidney<br />
 Lane 2: Mouse kidney<br />
 Lane 3: Human kidney<br />
 Lane 4: Hela
  • Western blot analysis of BMP2 on different lysates using anti-BMP2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat kidney<br />
 Lane 2: Mouse kidney<br />
 Lane 3: Human kidney<br />
 Lane 4: Hela
  • ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
  • ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of BMP2 on different lysates using anti-BMP2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat kidney
Lane 2: Mouse kidney
Lane 3: Human kidney
Lane 4: Hela

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BMP2 Antibody (ER80602)

Immunogen

Synthetic peptide (klh-coupled) within human bmp2 n terminal.

Host

Rabbit

Positive Control

MCF-7, Hela, mouse kidney tissue, human kidney tissue, rat kidney tissue, rat small intestine tissue, mouse small intestine tissue, human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

75kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

BMP2

SYNONYMS

BDA2 antibody; BMP-2 antibody; BMP-2A antibody; Bmp2 antibody; BMP2_HUMAN antibody; BMP2A antibody; Bone morphogenetic protein 2 antibody; Bone morphogenetic protein 2A antibody

SEQUENCE SIMILARITIES

Belongs to the TGF-beta family.

TISSUE SPECIFICITY

Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

SUBCELLULAR LOCATION

Secreted

FUNCTION

BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. Implantation of BMP-2 in a collagen sponge induces new bone formation and can be used for the treatment of bony defects, delayed union, and non-union.