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PRODUCT CODE: EM20602

Bmi1 Monoclonal Antibody (EM20602)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: 293T<br /> Lane 2: Jurkat<br /> Lane 3: Hela<br /> Lane 4: MCF-7<br /> Lane 5: HepG2<br /> Lane 6: NIH/3T3<br /> Lane 7: PC12<br /> Lane 8: Mouse kidney<br /> Lane 9: Human kidney<br /> Lane 10: K562<br /> Lane 11: Human brain
  • Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: 293T<br /> Lane 2: Jurkat<br /> Lane 3: Hela<br /> Lane 4: MCF-7<br /> Lane 5: HepG2<br /> Lane 6: NIH/3T3<br /> Lane 7: PC12<br /> Lane 8: Mouse kidney<br /> Lane 9: Human kidney<br /> Lane 10: K562<br /> Lane 11: Human brain
  • ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Jurkat
Lane 3: Hela
Lane 4: MCF-7
Lane 5: HepG2
Lane 6: NIH/3T3
Lane 7: PC12
Lane 8: Mouse kidney
Lane 9: Human kidney
Lane 10: K562
Lane 11: Human brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Bmi1 Monoclonal Antibody (EM20602)

Immunogen

Recombinant protein within human bmi1 full sequence.

Host

Mouse

Positive Control

Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, A549, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B3-G5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • ICC

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

P35226

PROTEIN NAME

Polycomb complex protein BMI-1

GENE NAME

BMI1

SYNONYMS

BMI1

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.