Positive control:
Lane 1: 293T
Lane 2: Jurkat
Lane 3: Hela
Lane 4: MCF-7
Lane 5: HepG2
Lane 6: NIH/3T3
Lane 7: PC12
Lane 8: Mouse kidney
Lane 9: Human kidney
Lane 10: K562
Lane 11: Human brain
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Bmi1 Monoclonal Antibody (EM20602)
Immunogen
Recombinant protein within human bmi1 full sequence.
Host
Mouse
Positive Control
Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, A549, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
B3-G5
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
37kDa
Isotype
IgG2a
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
ICC
-
1:200
-
FC
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
Bmi1
SYNONYMS
B lymphoma Mo MLV insertion region (mouse) antibody; B lymphoma Mo MLV insertion region 1 homolog antibody; Bmi 1 antibody; BMI1 antibody; BMI1 polycomb ring finger oncogene antibody; BMI1_HUMAN antibody; Flvi 2/bmi 1 antibody; FLVI2/BMI1 antibody; MGC12685 antibody; Murine leukemia viral (bmi 1) oncogene homolog antibody; Oncogene BMI 1 antibody; PCGF 4 antibody; PCGF4 antibody; Polycomb complex protein BMI 1 antibody; Polycomb complex protein BMI-1 antibody; Polycomb group protein Bmi1 antibody; Polycomb group ring finger 4 antibody; Polycomb group RING finger protein 4 antibody; RING finger protein 51 antibody; RNF 51 antibody; RNF51 antibody
POST-TRANSLATIONAL MODIFICATION
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm
FUNCTION
The Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest.