Positive control:
Lane 1: PC-3M cell lysate
Lane 2: MCF-7 cell lysate
Applications
-
WB
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Bit1 Antibody (ER2001-71)
Immunogen
Recombinant protein within human bit1 aa 1-179.
Host
Rabbit
Positive Control
PC-3M cell lysate, MCF-7 cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
Predicted band size: 13/19 kDa.
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
TARGET
UNIPROT #
PROTEIN NAME
Bit1
SYNONYMS
Bcl 2 inhibitor of transcription 1 antibody; Bcl-2 inhibitor of transcription 1 antibody; BIT 1 antibody; BIT1 antibody; CGI 147 antibody; CGI147 antibody; mitochondrial antibody; Peptidyl tRNA hydrolase 2 mitochondrial antibody; Peptidyl-tRNA hydrolase 2 antibody; PTH 2 antibody; PTH2 antibody; PTH2_HUMAN antibody; PTRH 2 antibody; PTRH2 antibody
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.