PRODUCT CODE: ER2001-71

Bit1 Antibody (ER2001-71)

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis of Bit1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: PC-3M cell lysate<br />
Lane 2: MCF-7 cell lysate
  • Western blot analysis of Bit1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: PC-3M cell lysate<br />
Lane 2: MCF-7 cell lysate
Western blot analysis of Bit1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-3M cell lysate
Lane 2: MCF-7 cell lysate

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Bit1 Antibody (ER2001-71)

Immunogen

Recombinant protein within human bit1 aa 1-179.

Host

Rabbit

Positive Control

PC-3M cell lysate, MCF-7 cell lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size: 13/19 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Peptidyl-tRNA hydrolase 2, mitochondrial PTH 2 Bcl-2 inhibitor of transcription 1

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.