PRODUCT CODE: ER0805

Beta-Catenin Antibody (ER0805)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Beta-catenin on different lysates using anti-Beta-catenin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: A431<br />
Lane 2: SW480<br />
Lane 3: HCT116<br />
Lane 4: HT-29<br />
Lane 5: Mouse colon<br />
Lane 6: Mouse brain
  • Western blot analysis of Beta-catenin on different lysates using anti-Beta-catenin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: A431<br />
Lane 2: SW480<br />
Lane 3: HCT116<br />
Lane 4: HT-29<br />
Lane 5: Mouse colon<br />
Lane 6: Mouse brain
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Beta-catenin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Beta-catenin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Beta-catenin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of Beta-catenin on different lysates using anti-Beta-catenin antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: SW480
Lane 3: HCT116
Lane 4: HT-29
Lane 5: Mouse colon
Lane 6: Mouse brain

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Beta-Catenin Antibody (ER0805)

Immunogen

Synthetic peptide (klh-coupled) witihin human beta-catenin c terminal.

Host

Rabbit

Positive Control

A431, SW480, HCT116, HT-29, mouse colon Mouse brain, human lung carcinoma tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

85kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Catenin beta-1

GENE NAME

CTNNB1

SYNONYMS

CTNB1

SEQUENCE SIMILARITIES

Belongs to the beta-catenin family.

TISSUE SPECIFICITY

Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.; Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. Ubiquitinated and degraded following interaction with SOX9 (By similarity).; S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions.; O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1.; Deacetylated at Lys-49 by SIRT1.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse.

FUNCTION

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).