PRODUCT CODE: EM21002

Beta Actin Monoclonal Antibody (EM21002)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3<br />
Lane 2: PC12<br />
Lane 3: MCF-7<br />
Lane 4: HepG2<br />
Lane 5: Hela<br />
Lane 6: Mouse lung
  • Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3<br />
Lane 2: PC12<br />
Lane 3: MCF-7<br />
Lane 4: HepG2<br />
Lane 5: Hela<br />
Lane 6: Mouse lung
  • ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: PC12
Lane 3: MCF-7
Lane 4: HepG2
Lane 5: Hela
Lane 6: Mouse lung

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Beta Actin Monoclonal Antibody (EM21002)

Immunogen

Synthetic peptide (klh-coupled) within human beta-actin n terminal.

Host

Mouse

Positive Control

NIH/3T3, PC12, MCF-7, HepG2, Hela, mouse lung A549, human colon carcinoma tissue, mouse prostate tissue , mouse kidney tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:10,000

  • ICC

  • 1:100-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Actin, cytoplasmic 1

GENE NAME

ACTB

SYNONYMS

ACTB

SEQUENCE SIMILARITIES

Belongs to the actin family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

FUNCTION

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

CITATIONS

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    Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress. | Cell Stress & Chaperones [2012]

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    Molecular cloning and characterization of juvenile hormone acid methyltransferase in the honey bee, Apis mellifera, and its differential expression during caste differentiation. | PloS One [2013]

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    Activation of cAMP-response element-binding protein is positively regulated by PKA and calcium-sensitive calcineurin and negatively by PKC in insect. | Insect Biochemistry and Molecular Biology [2013]

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    Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis. | Gene [2014]

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    Differential regulation of the ten-eleven translocation (TET) family of dioxygenases by O-linked �_-N-acetylglucosamine transferase (OGT). | The Journal of Biological Chemistry [2014]

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    Niacin activates the PI3K/Akt cascade via PKC- and EGFR-transactivation-dependent pathways through hydroxyl-carboxylic acid receptor 2. | PloS One [2014]

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    Effect of Huazhuojiedu medicated serum on the proliferation and activation of hepatic stellate cells and the expression of PI3K and p-Akt in rats. | International Journal of Clinical and Experimental Medicine [2014]

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    Cav1.2 of L-type Calcium Channel Is a Key Factor for the Differentiation of Dental Pulp Stem Cells. | Journal of Endodontics [2015]

  • Wang, Qian et al.

    Myricetin suppresses differentiation of 3 T3-L1 preadipocytes and enhances lipolysis in adipocytes. | Nutrition Research (New York, N.Y.) [2015]

  • Zheng, Shuli et al.

    Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia. | Experimental Neurology [2015]

  • Jingwei, Chen et al.

    Effect of Bushenwenyanghuayu decoction on nerve growth factor and bradykinin/bradykinin B1 receptor in a endometriosis dysmenorrhea mouse model. | Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan [2015]

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    Effective components of Chinese herbs reduce central nervous system function decline induced by iron overload. | Neural Regeneration Research [2015]

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    Hawthorn leaf flavonoids alleviate nonalcoholic fatty liver disease by enhancing the adiponectin/AMPK pathway. | International Journal of Clinical and Experimental Medicine [2015]

  • Shan, Ying et al.

    Matrix metalloproteinase-9 plays a role in protecting zebrafish from lethal infection with Listeria monocytogenes by enhancing macrophage migration. | Fish & Shellfish Immunology [2016]

  • Xu, Yunbin et al.

    The co-chaperone Cdc37 regulates the rabies virus phosphoprotein stability by targeting to Hsp90AA1 machinery. | Scientific Reports [2016]

  • Gao, Fei et al.

    Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism. | Scientific Reports [2016]

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    Mechanisms and metabolic regulation of PPAR� activation in Nile tilapia (Oreochromis niloticus). | Biochimica Et Biophysica Acta [2016]

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    Size-dependent cytotoxicity of Fe3O4 nanoparticles induced by biphasic regulation of oxidative stress in different human hepatoma cells. | International Journal of Nanomedicine [2016]

  • Wang, Quanquan et al.

    The Relationship between the Bcl-2/Bax Proteins and the Mitochondria-Mediated Apoptosis Pathway in the Differentiation of Adipose-Derived Stromal Cells into Neurons. | PloS One [2016]

  • Liu, Han et al.

    Quantitative Proteomic Analysis of Germination of Nosema bombycis Spores under Extremely Alkaline Conditions. | Frontiers in Microbiology [2016]

  • Yang, Xiao-Pei et al.

    EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis. | Journal of Neuroinflammation [2016]

  • Guo, Chuansheng et al.

    Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome. | Immunity [2016]

  • Chen, Hao et al.

    The Bacterial T6SS Effector EvpP Prevents NLRP3 Inflammasome Activation by Inhibiting the Ca2+-Dependent MAPK-Jnk Pathway. | Cell Host & Microbe [2017]

  • Xiang, Bingyu et al.

    Transplantation of Menstrual Blood-Derived Mesenchymal Stem Cells Promotes the Repair of LPS-Induced Acute Lung Injury. | International Journal of Molecular Sciences [2017]

  • Cai, Chenlei et al.

    PLGA-based dual targeted nanoparticles enhance miRNA transfection efficiency in hepatic carcinoma. | Scientific Reports [2017]

  • Huang, Wan et al.

    Promoter mutations and cellular distribution of telomerase in non-clear cell and clear cell hepatocellular carcinoma. | Oncotarget [2017]

  • Li, H et al.

    Smurf1 regulates lung cancer cell growth and migration through interaction with and ubiquitination of PIPKI�_. | Oncogene [2017]

  • Xu, Xiu-Ping et al.

    Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro. | Stem Cell Research & Therapy [2017]

  • Shu, Xiangbing et al.

    Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-�B Activation. | Evidence-Based Complementary and Alternative Medicine : ECam [2017]

  • Zhao, Xibao et al.

    Teuvincenone F Suppresses LPS-Induced Inflammation and NLRP3 Inflammasome Activation by Attenuating NEMO Ubiquitination. | Frontiers in Pharmacology [2017]

  • Chen, Yingzhu et al.

    Necrostatin-1 Improves Long-term Functional Recovery Through Protecting Oligodendrocyte Precursor Cells After Transient Focal Cerebral Ischemia in Mice. | Neuroscience [2018]

  • Wang, Zheng et al.

    Overexpression of ceramide synthase 1 increases C18-ceramide and leads to lethal autophagy in human glioma. | Oncotarget [2017]

  • Zhang, Biyan et al.

    Antineoplastic activity of isoliquiritigenin, a chalcone compound, in androgen-independent human prostate cancer cells linked to G2/M cell cycle arrest and cell apoptosis. | European Journal of Pharmacology [2018]

  • Wu, Weiche et al.

    Muscle-specific deletion of Prkaa1 enhances skeletal muscle lipid accumulation in mice fed a high-fat diet. | Journal of Physiology and Biochemistry [2018]

  • Cong, Xiao Xia et al.

    Activation of AKT-mTOR Signaling Directs Tenogenesis of Mesenchymal Stem Cells. | Stem Cells (Dayton, Ohio) [2018]

  • Ge, Jiafeng et al.

    Ubiquitin carboxyl-terminal hydrolase isozyme L5 inhibits human glioma cell migration and invasion via downregulating SNRPF. | Oncotarget [2017]

  • Sun, Dawei et al.

    bFGF plays a neuroprotective role by suppressing excessive autophagy and apoptosis after transient global cerebral ischemia in rats. | Cell Death & Disease [2018]

  • Wang, Xuan et al.

    A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis. | Oncology Letters [2018]

  • Chen, Ao et al.

    Liraglutide attenuates NLRP3 inflammasome-dependent pyroptosis via regulating SIRT1/NOX4/ROS pathway in H9c2 cells. | Biochemical and Biophysical Research Communications [2018]

  • Wang, Jing et al.

    The impact of acute thermal stress on green mussel Perna viridis: Oxidative damage and responses. | Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology [2018]

  • Cao, Wei et al.

    Gamma Oscillation Dysfunction in mPFC Leads to Social Deficits in Neuroligin 3 R451C Knockin Mice. | Neuron [2018]

  • Zhang, Yuefeng et al.

    Pancreatic cancer-derived exosomes suppress the production of GIP and GLP-1 from STC-1���cells in vitro by down-regulating the PCSK1/3. | Cancer Letters [2018]

  • Shan, Ying et al.

    Nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-�� production by blocking the nuclear factor-�B nuclear translocation. | Journal of Zhejiang University. Science. B [2018]

  • Chen, Haiqun et al.

    Vasodilator-stimulated phosphoprotein (VASP), a novel target of miR-4455, promotes gastric cancer cell proliferation, migration, and invasion, through activating the PI3K/AKT signaling pathway. | Cancer Cell International [2018]

  • Wu, Huansheng et al.

    Ubiquitination Is Essential for Avibirnavirus Replication by Supporting VP1 Polymerase Activity. | Journal of Virology [2019]

  • Xie, Jiamin et al.

    Involvement of follistatin-like 3 in preeclampsia. | Biochemical and Biophysical Research Communications [2018]

  • Jin, Xing et al.

    An essential role for GLUT5-mediated fructose utilization in exacerbating the malignancy of clear cell renal cell carcinoma. | Cell Biology and Toxicology [2019]

  • Miao, Lu-Jie et al.

    miR-106b promotes proliferation and invasion by targeting Capicua through MAPK signaling in renal carcinoma cancer. | OncoTargets and Therapy [2019]

  • Li, Z et al.

    Growth hormone-releasing hormone promotes therapeutic effects of peripheral blood endothelial progenitor cells in ischemic repair. | Journal of Endocrinological Investigation [2020]

  • Xu, Jing et al.

    Neuroligin 3 Regulates Dendritic Outgrowth by Modulating Akt/mTOR Signaling. | Frontiers in Cellular Neuroscience [2019]

  • Wang, Zhijun et al.

    MiRNA-130a-3p inhibits cell proliferation, migration, and TMZ resistance in glioblastoma by targeting Sp1. | American Journal of Translational Research [2019]

  • Wang, Zhijun et al.

    MiRNA-130a-3p inhibits cell proliferation, migration, and TMZ resistance in glioblastoma by targeting Sp1. | American Journal of Translational Research [2019]

  • Wang, Yixuan et al.

    Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy. | Annals of Translational Medicine [2020]

  • Xiang, Qian et al.

    Gerontoxanthone I and Macluraxanthone Induce Mitophagy and Attenuate Ischemia/Reperfusion Injury. | Frontiers in Pharmacology [2020]