PRODUCT CODE: 0807-2

beta III Tubulin Antibody (0807-2)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of beta III Tubulin on PC-12 (1) and Hela (2) cell lysate using anti-beta III Tubulin antibody at 1/2,000 dilution.
  • Western blot analysis of beta III Tubulin on PC-12 (1) and Hela (2) cell lysate using anti-beta III Tubulin antibody at 1/2,000 dilution.
Western blot analysis of beta III Tubulin on PC-12 (1) and Hela (2) cell lysate using anti-beta III Tubulin antibody at 1/2,000 dilution.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

beta III Tubulin Antibody (0807-2)

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (klh-coupled) corresponding to near c-terminal residues of human beta iii tubulin.

Host

Rabbit

Positive Control

Hela, PC12

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Tubulin beta-3 chain

GENE NAME

TUBB3

SYNONYMS

TUBB3

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

TISSUE SPECIFICITY

Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, filopodium.

FUNCTION

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.

CITATIONS

  • Lin, Cui et al.

    Caspase-Dependent Apoptosis Induction via Viral Protein ORF4 of Porcine Circovirus 2 Binding to Mitochondrial Adenine Nucleotide Translocase 3. | Journal of Virology [2018]