PRODUCT CODE: EM1701-82

BCL2 Monoclonal Antibody (EM1701-82)

  • IVD–IHC

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of BCL2 on THP-1 lysate using anti-BCL2 antibody at 1/500 dilution.
  • Western blot analysis of BCL2 on THP-1 lysate using anti-BCL2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
Western blot analysis of BCL2 on THP-1 lysate using anti-BCL2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

BCL2 Monoclonal Antibody (EM1701-82)

Immunogen

Synthetic peptide within human bcl2 35-60 aa.

Host

Mouse

Positive Control

THP-1, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9F1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Apoptosis regulator Bcl-2

GENE NAME

BCL2

SYNONYMS

BCL2

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a variety of tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

FUNCTION

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.