PRODUCT CODE: ER1802-86

BCHE Rabbit Polyclonal Antibody (ER1802-86)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.
  • Western blot analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.
  • ICC staining BCHE in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BCHE in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BCHE antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-BCHE antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-BCHE antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BCHE antibody. Counter stained with hematoxylin.
Western blot analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BCHE Rabbit Polyclonal Antibody (ER1802-86)

Immunogen

Recombinant protein within human bche aa 300-500.

Host

Rabbit

Positive Control

Human blood plasma, HepG2, LOVO, human liver tissue, human colon cancer tissue, human skin tissue, human prostate tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

BCHE

SYNONYMS

Acylcholine acylhydrolase antibody; BCHE antibody; Butyrylcholine esterase antibody; CHE1 antibody; CHE2 antibody; CHLE_HUMAN antibody; Choline esterase II antibody; Cholinesterase (serum) 2 antibody; Cholinesterase 1 antibody; Cholinesterase antibody; E1 antibody; Pseudocholinesterase antibody; Pseudocholinesterase E1 antibody

SEQUENCE SIMILARITIES

Belongs to the type-B carboxylesterase/lipase family.

TISSUE SPECIFICITY

Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. No other PTM detected. The major N-glycan structures are of the complex diantennary type with 1 and 2 N-acetylneuraminic acid molecules (Neu5Ac) making up approximately 33% and 47% of the total N-glycans, respectively. Only low amounts of fucosylated diantennary N-glycans are detected (approximately 2%). Triantennary N-glycans with or without fucose amount to approximately 13%, whereas 5% of the total N-glycans are of the oligomannosidic or hybrid type.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Present in most cells except erythrocytes, butyrylcholine esterase (BChE), also designated acylcholine acylhydrolase or pseudocholinesterase, has esterase activity as well as aryl acylamidase activity. It hydrolyzes acylcholine into choline and carboxylate. BChE is synthesized in the liver and is highly reactive with organophosphate esters. BChE can form a homotetramer composed of two dimers linked by a disulfide bond. Defects in the gene encoding BChE are associated with the disease hypocholinesterasemia. Inhibition of BChE effects the toxicity of organophosphates in the respiratory system suggesting that BChE may play a role in respiratory function. In addition, BChE may play an important pharmocological role by hydrolyzing toxic esters. This suggests an involvement of BChE in a treatment for intoxication with substances such as cocaine.