PRODUCT CODE: EM1203

BAX Monoclonal Antibody (EM1203)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Raji<br />
 Lane 2: Mouse lymphoid tissue
  • Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Raji<br />
 Lane 2: Mouse lymphoid tissue
  • ICC staining BAX in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using BAX antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using BAX antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgA (FITC) was used as the secondary antibody.
Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution.
Positive control:
Lane 1: Raji
Lane 2: Mouse lymphoid tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

BAX Monoclonal Antibody (EM1203)

Immunogen

This antibody is produced by immunizing mice with a synthetic peptide (klh-coupled) corresponding to n-terminal bax.

Host

Mouse

Positive Control

Raji, mouse lymphoid tissue, HepG2, human gallbladder tissue, mouse testis tissue, Hela

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-D2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgA

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

BAX

SYNONYMS

Apoptosis regulator BAX antibody; BAX antibody; Bax-protein antibody; BAX_HUMAN antibody; BAXA antibody; Baxdelta2G9 antibody; Baxdelta2G9omega antibody; Baxdelta2omega antibody; Bcl-2-like protein 4 antibody; BCL2 associated X protein antibody; BCL2 associated X protein omega antibody; BCL2 associated X protein transcript variant delta2 antibody; Bcl2-L-4 antibody; BCL2L4 antibody; membrane isoform alpha antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

SUBCELLULAR LOCATION

Mitochondrion membrane, cytoplasm

FUNCTION

BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.

CITATIONS

  • Xie, Cai-Jun et al.

    Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke. | Brain and Behavior [2018]