PRODUCT CODE: ER1802-56

B7-H4(VTCN1) Antibody (ER1802-56)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of B7-H4(VTCN1) on SKBR3 cell and human colon tissue lysates using anti-B7-H4(VTCN1) antibody at 1/500 dilution.
  • Western blot analysis of B7-H4(VTCN1) on SKBR3 cell and human colon tissue lysates using anti-B7-H4(VTCN1) antibody at 1/500 dilution.
  • ICC staining B7-H4(VTCN1) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with B7-H4(VTCN1) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of B7-H4(VTCN1) on SKBR3 cell and human colon tissue lysates using anti-B7-H4(VTCN1) antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

B7-H4(VTCN1) Antibody (ER1802-56)

Immunogen

Recombinant protein with human b7-h4 aa 50-220.

Host

Rabbit

Positive Control

SKBR3 cell and human colon tissue lysates, MCF-7, rat epididymid tissue, human breast tissue, human breast tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

31/21/10 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

V-set domain-containing T-cell activation inhibitor 1

GENE NAME

VTCN1

SYNONYMS

B7-H4, VTCN1, B7H4

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. BTN/MOG family.

TISSUE SPECIFICITY

Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.