PRODUCT CODE: 0908-2

Autoimmune regulator Antibody (0908-2)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Mouse

Western blot analysis on 293 cell (A) transfected and (B) none transfected with AIRE gene using anti-AIRE polyclonal antibody.
  • Western blot analysis on 293 cell (A) transfected and (B) none transfected with AIRE gene using anti-AIRE polyclonal antibody.
  • Immunohistochemistry- staining AIRE in mouse thymus by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • AIRE staining (red) in mouse embryonic stem cells (D3) by immunocytochemistry, nuclear staining with DAPI (blue) and SSEA-1 staining (green).
Western blot analysis on 293 cell (A) transfected and (B) none transfected with AIRE gene using anti-AIRE polyclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Autoimmune regulator Antibody (0908-2)

Immunogen

Peptide

Host

Rabbit

Positive Control

Transfected 293 cells

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:500

  • IHC-P

  • 1:100

TARGET

UNIPROT #

PROTEIN NAME

Autoimmune regulator

GENE NAME

Aire

SYNONYMS

APECED protein homolog, Aire

TISSUE SPECIFICITY

Highly expressed in a few cells in the medulla of the thymus (medullary epithelial cells) (at protein level). Expressed in thymic but no peripheral B-cells. In secondary lymphoid organs, expressed in a discrete population of bone marrow-derived toleregenic antigen presenting cells (APCs) called extrathymic AIRE expressing cells (eTAC)(at protein level). Detected at very low levels in thymus, lymph node, liver, brain, ovary, lung, testis, kidney, heart, spleen, bone marrow, skeletal muscle and adrenal gland. Isoforms 1a to 1d predominate, isoforms 2a to 2d are intermediate and isoforms 3a to 3d are expressed at extremely low levels.

DEVELOPMENTAL STAGE

In the thymus, not expressed at 13.5 dpc but present at 16.5 dpc and postnatal day 1.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Note=Predominantly nuclear but also cytoplasmic. Found in nuclear body-like structures (dots) and in a filamentous vimentin-like pattern.

FUNCTION

Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (Probable). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (By similarity). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli.