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PRODUCT CODE: ER1803-34

ATP2A1 Antibody (ER1803-34)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Mouse skeletal muscle tissue <br /> Lane 2: Human skeletal muscle tissue<br />
  • Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Mouse skeletal muscle tissue <br /> Lane 2: Human skeletal muscle tissue<br />
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with ATP2A1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse skeletal muscle tissue
Lane 2: Human skeletal muscle tissue

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ATP2A1 Antibody (ER1803-34)

Immunogen

Synthetis peptide within rat atp2a1 aa 20-50.

Host

Rabbit

Positive Control

Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, 293T.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

110 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O14983

PROTEIN NAME

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

GENE NAME

ATP2A1

SYNONYMS

SERCA1, SR Ca(2+)-ATPase 1, ATP2A1

SEQUENCE SIMILARITIES

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

TISSUE SPECIFICITY

Skeletal muscle, fast twitch muscle (type II) fibers.

DEVELOPMENTAL STAGE

Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

FUNCTION

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction.