PRODUCT CODE: ER1802-82

Atg12 Antibody (ER1802-82)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: A431<br />
 Lane 2: Human kidney tissue<br />
 Lane 3: 293T<br />
  • Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: A431<br />
 Lane 2: Human kidney tissue<br />
 Lane 3: 293T<br />
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Human kidney tissue
Lane 3: 293T

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Atg12 Antibody (ER1802-82)

Immunogen

Recombinant protein within human atg12 aa 1-100.

Host

Rabbit

Positive Control

A431, human kidney tissue lysate, 293T, human liver cancer tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

8/15 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin-like protein ATG12

GENE NAME

ATG12

SYNONYMS

APG12-like, ATG12, APG12, APG12L

SEQUENCE SIMILARITIES

Belongs to the ATG12 family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Acetylated by EP300.

SUBCELLULAR LOCATION

Cytoplasm; Peripheral membrane protein. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.

FUNCTION

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.; (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established.