PRODUCT CODE: em1701-94

ATF6 Monoclonal Antibody (EM1701-94)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.
  • Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with ATF6 antibody at 1/50 dilution (pink purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ATF6 Monoclonal Antibody (EM1701-94)

Immunogen

Recombinant protein within full length sequence of human atf6.

Host

Mouse

Positive Control

SiHa, rat brain tissue, human placenta tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8D3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Cyclic AMP-dependent transcription factor ATF-6 alpha

GENE NAME

ATF6

SYNONYMS

cAMP-dependent transcription factor ATF-6 alpha, ATF6-alpha, ATF6

SEQUENCE SIMILARITIES

Belongs to the bZIP family. ATF subfamily.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.; N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).; Phosphorylated in vitro by MAPK14/P38MAPK.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Single-pass type II membrane protein.; [Processed cyclic AMP-dependent transcription factor ATF-6 alpha]: Nucleus. Note=Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.

FUNCTION

Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.