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PRODUCT CODE: ER1802-38

Aromatase Antibody (ER1802-38)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution.
  • Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution.
  • ICC staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Aromatase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Aromatase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Aromatase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Aromatase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Aromatase Antibody (ER1802-38)

Immunogen

Peptide

Host

Rabbit

Positive Control

HepG2, SH-SY-5Y, SK-Br-3, human placenta tissue, mouse cerebellum tissue lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P11511

PROTEIN NAME

Aromatase

SYNONYMS

ARO antibody; ARO1 antibody; Aromatase antibody; CP19A_HUMAN antibody; CPV1 antibody; CYAR antibody; CYP19 antibody; Cyp19a1 antibody; CYPXIX antibody; Cytochrome P-450AROM antibody; Cytochrome P450 19A1 antibody; Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody; Cytochrome P450, subfamily XIX (aromatization of androgens) antibody; Estrogen synthase antibody; Estrogen synthetase antibody; Flavoprotein linked monooxygenase antibody; MGC104309 antibody; Microsomal monooxygenase antibody; OTTHUMP00000162543 antibody; OTTHUMP00000198350 antibody; P 450AROM antibody

SEQUENCE SIMILARITIES

Belongs to the cytochrome P450 family.

TISSUE SPECIFICITY

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).

SUBCELLULAR LOCATION

Membrane.

FUNCTION

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased CYP19 activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7α-hydroxylase, CYP7A1, is the rate-limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver.