PRODUCT CODE: ER1802-47

APOC3 Antibody (ER1802-47)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of APOC3 on human liver tissue lysate using anti-APOC3 antibody at 1/500 dilution.
  • Western blot analysis of APOC3 on human liver tissue lysate using anti-APOC3 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APOC3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with APOC3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of APOC3 on human liver tissue lysate using anti-APOC3 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

APOC3 Antibody (ER1802-47)

Immunogen

Recombinant protein with human apoc3 aa 1-100.

Host

Rabbit

Positive Control

Human liver tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Apolipoprotein C-III

GENE NAME

APOC3

SYNONYMS

Apo-CIII, ApoC-III

SEQUENCE SIMILARITIES

Belongs to the apolipoprotein C3 family.

TISSUE SPECIFICITY

Liver.

POST-TRANSLATIONAL MODIFICATION

The most abundant glycoforms are characterized by an O-linked disaccharide galactose linked to N-acetylgalactosamine (Gal-GalNAc), further modified with up to 3 sialic acid residues. Less abundant glycoforms are characterized by more complex and fucosylated glycan moieties. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.