PRODUCT CODE: ER1802-71

Apg3 Antibody (ER1802-71)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Apg3 on HL-60 cell lysate using anti-Apg3 antibody at 1/500 dilution.
  • Western blot analysis of Apg3 on HL-60 cell lysate using anti-Apg3 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Apg3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Apg3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Apg3 antibody. Counter stained with hematoxylin.
Western blot analysis of Apg3 on HL-60 cell lysate using anti-Apg3 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Apg3 Antibody (ER1802-71)

Immunogen

Recombinant protein with human apg3 aa 50-250.

Host

Rabbit

Positive Control

HL-60, human tonsil tissue, human thyroid tissue, human colon cancer tissue, human small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Apg3

SYNONYMS

2610016C12Rik antibody; Apg 3 antibody; APG3 antibody; APG3 autophagy 3 like antibody; APG3 like antibody; APG3, S. cerevisiae, homolog of antibody; APG3-like antibody; APG3L antibody; Apg3p antibody; ATG 3 antibody; ATG3 antibody; ATG3 autophagy related 3 homolog antibody; ATG3 autophagy related 3 homolog (S. cerevisiae) antibody; ATG3_HUMAN antibody; Autophagy 3, S. cerevisiae, homolog of antibody; Autophagy Apg3p/Aut1p like antibody; autophagy related 3 antibody; Autophagy related protein 3 antibody; Autophagy-related protein 3 antibody; DKFZp564M1178 antibody; FLJ22125 antibody; hApg3 antibody; MGC15201 antibody; OTTHUMP00000214547 antibody; OTTHUMP00000214548 antibody; PC3 96 antibody; Protein PC3-96 antibody; Ubiquitin-like-conjugating enzyme ATG3 antibody

SEQUENCE SIMILARITIES

Belongs to the ATG3 family.

TISSUE SPECIFICITY

Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.

POST-TRANSLATIONAL MODIFICATION

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.; Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Atg3 (autophagy-related protein 3), also known as APG3-like, hAPG3 or PC3-96, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. Atg3 catalyzes the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, a reaction that is essential for autophagy (a cellular process that allows for the degradation of organelles and bulk cellular proteins). The process of forming the Atg8-PE conjugate begins with the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease. The, now exposed, glycine residue is then activated by Atg7 and is then transferred to Atg3 for the final conjugation to PE. This last step can be accelerated by the presence of the Atg12-Atg5 conjugate which functions similarly to an E3 enzyme.