Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
APC Antibody (ER1802-39)
Immunogen
Peptide
Host
Rabbit
Positive Control
HUVEC, SH-SY-5Y, SiHa, rat brain tissue, human kidney tissue, human breast tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
Predicted band size 312 kDa
Isotype
IgG
APPLICATION DILUTION
-
ICC
-
1:50-1:200
-
IHC-P
-
1:100-1:200
-
FC
-
1:50-1:100
-
WB
-
1:500
TARGET
UNIPROT #
PROTEIN NAME
APC
SYNONYMS
Adenomatous Polyposis Coli antibody; Adenomatous polyposis coli protein antibody; Apc antibody; APC_HUMAN antibody; CC1 antibody; Deleted in polyposis 2.5 antibody; DP2 antibody; DP2.5 antibody; DP3 antibody; FAP antibody; FPC antibody; GS antibody; Protein APC antibody
SEQUENCE SIMILARITIES
Belongs to the adenomatous polyposis coli (APC) family.
TISSUE SPECIFICITY
Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver. Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues. Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by GSK3B.; Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
SUBCELLULAR LOCATION
Cell junction. Cytoplasm. Cytoskeleton. Membrane.
FUNCTION
The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. β-catenin and γ-catenin (also called plakoglobin), are involved in the regulation of cellular adhesion. APC and E-cadherin compete for binding to specific internal regions of both β- and γ-catenin. Interactions between cytoskeleton and the APC, E-cadherin, β/γ catenin complex are mediated by α-catenin.