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PRODUCT CODE: ER1802-39

APC Antibody (ER1802-39)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-APC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-APC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-APC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-APC antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with APC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

APC Antibody (ER1802-39)

Immunogen

Peptide

Host

Rabbit

Positive Control

HUVEC, SH-SY-5Y, SiHa, rat brain tissue, human kidney tissue, human breast tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

Predicted band size 312 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

P25054

PROTEIN NAME

APC

SYNONYMS

Adenomatous Polyposis Coli antibody; Adenomatous polyposis coli protein antibody; Apc antibody; APC_HUMAN antibody; CC1 antibody; Deleted in polyposis 2.5 antibody; DP2 antibody; DP2.5 antibody; DP3 antibody; FAP antibody; FPC antibody; GS antibody; Protein APC antibody

SEQUENCE SIMILARITIES

Belongs to the adenomatous polyposis coli (APC) family.

TISSUE SPECIFICITY

Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver. Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues. Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by GSK3B.; Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.

SUBCELLULAR LOCATION

Cell junction. Cytoplasm. Cytoskeleton. Membrane.

FUNCTION

The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. β-catenin and γ-catenin (also called plakoglobin), are involved in the regulation of cellular adhesion. APC and E-cadherin compete for binding to specific internal regions of both β- and γ-catenin. Interactions between cytoskeleton and the APC, E-cadherin, β/γ catenin complex are mediated by α-catenin.