PRODUCT CODE: ER1802-50

Annexin A3 Antibody (ER1802-50)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: Siha <br />
  Lane 2: Hela<br />
  Lane 3: Rat spleen tissue<br />
  Lane 4: Rat liver tissue<br />
  Lane 5: Mouse lung tissue
  • Western blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: Siha <br />
  Lane 2: Hela<br />
  Lane 3: Rat spleen tissue<br />
  Lane 4: Rat liver tissue<br />
  Lane 5: Mouse lung tissue
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with Annexin A3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Siha
Lane 2: Hela
Lane 3: Rat spleen tissue
Lane 4: Rat liver tissue
Lane 5: Mouse lung tissue

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Annexin A3 Antibody (ER1802-50)

Immunogen

Recombinant protein with human annexin a3 aa 1-350.

Host

Rabbit

Positive Control

Siha, Hela, rat spleen tissue lysate, rat liver tissue lysate, mouse lung tissue lysate, rat kidney tissue, human colon cancer tissue, human spleen tissue, human prostate tissue, A431.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Annexin A3

SYNONYMS

2-cyclic phosphate 2-phosphohydrolase antibody; 35-alpha calcimedin antibody; Annexin A3 antibody; Annexin III antibody; Annexin-3 antibody; ANX3 antibody; ANXA 3 antibody; Anxa3 antibody; ANXA3_HUMAN antibody; Calcimedin 35 alpha antibody; Inositol 1 2 cyclic phosphate 2 phosphohydrolase antibody; Inositol 1 antibody; Lipocortin III antibody; OTTHUMP00000160701 antibody; OTTHUMP00000219237 antibody; OTTHUMP00000219238 antibody; PAP III antibody; PAP-III antibody; Placental anticoagulant protein III antibody

SEQUENCE SIMILARITIES

Belongs to the annexin family.

SUBCELLULAR LOCATION

Cytoplasm. Secreted. Plasma Membrane.

FUNCTION

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-terminal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis, exocytosis and cellular adhesion. Annexin III, also known as ANXA3, Annexin A3, Lipocortin III, PAP-III (placental anticoagulant protein III), 35-α calcimedin or ANX3, belongs to the annexin family and contains four Annexin repeats. Annexin III exhibits a variety of functions, including anti-coagulent properties, an inhibitory role towards phospholipase A2 (PLA2) and an enzymatic function, cleaving the cyclic bond of inositol 1,2-cyclic phosphate to produce inositol 1-phosphate.